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e coli bl21 strain  (New England Biolabs)


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    New England Biolabs e coli bl21 strain
    FRET constructs and ratio (A) Plasmid designs of various constructs are illustrated. The sequences of three linkers and two tandem repeats (TR) proteins used in the study are shown. (B) Representative image of E. coli cultures expressing each construct. (C) Scatterplot of fluorescence intensity obtained in the green and red channels with the plate reader. The dotted and solid circles denote two biological replicates per sample with eight technical replicates each. FRET ratios measured by HyCAP are significantly higher than those determined by the plate reader, owing to differences in the excitation and emission measurement setups. FRET ratio distributions of replicates measured using plate reader (30 replicates each) (D) and HyCAP (E).
    E Coli Bl21 Strain, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4872 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+bl21+strain/pmc13049519-2-0-5?v=New+England+Biolabs
    Average 99 stars, based on 4872 article reviews
    e coli bl21 strain - by Bioz Stars, 2026-07
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    1) Product Images from "High-throughput screening of FRET-based proteins using a hyperspectral microcapillary array"

    Article Title: High-throughput screening of FRET-based proteins using a hyperspectral microcapillary array

    Journal: iScience

    doi: 10.1016/j.isci.2026.115302

    FRET constructs and ratio (A) Plasmid designs of various constructs are illustrated. The sequences of three linkers and two tandem repeats (TR) proteins used in the study are shown. (B) Representative image of E. coli cultures expressing each construct. (C) Scatterplot of fluorescence intensity obtained in the green and red channels with the plate reader. The dotted and solid circles denote two biological replicates per sample with eight technical replicates each. FRET ratios measured by HyCAP are significantly higher than those determined by the plate reader, owing to differences in the excitation and emission measurement setups. FRET ratio distributions of replicates measured using plate reader (30 replicates each) (D) and HyCAP (E).
    Figure Legend Snippet: FRET constructs and ratio (A) Plasmid designs of various constructs are illustrated. The sequences of three linkers and two tandem repeats (TR) proteins used in the study are shown. (B) Representative image of E. coli cultures expressing each construct. (C) Scatterplot of fluorescence intensity obtained in the green and red channels with the plate reader. The dotted and solid circles denote two biological replicates per sample with eight technical replicates each. FRET ratios measured by HyCAP are significantly higher than those determined by the plate reader, owing to differences in the excitation and emission measurement setups. FRET ratio distributions of replicates measured using plate reader (30 replicates each) (D) and HyCAP (E).

    Techniques Used: Construct, Plasmid Preparation, Expressing, Fluorescence



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    Image Search Results


    FRET constructs and ratio (A) Plasmid designs of various constructs are illustrated. The sequences of three linkers and two tandem repeats (TR) proteins used in the study are shown. (B) Representative image of E. coli cultures expressing each construct. (C) Scatterplot of fluorescence intensity obtained in the green and red channels with the plate reader. The dotted and solid circles denote two biological replicates per sample with eight technical replicates each. FRET ratios measured by HyCAP are significantly higher than those determined by the plate reader, owing to differences in the excitation and emission measurement setups. FRET ratio distributions of replicates measured using plate reader (30 replicates each) (D) and HyCAP (E).

    Journal: iScience

    Article Title: High-throughput screening of FRET-based proteins using a hyperspectral microcapillary array

    doi: 10.1016/j.isci.2026.115302

    Figure Lengend Snippet: FRET constructs and ratio (A) Plasmid designs of various constructs are illustrated. The sequences of three linkers and two tandem repeats (TR) proteins used in the study are shown. (B) Representative image of E. coli cultures expressing each construct. (C) Scatterplot of fluorescence intensity obtained in the green and red channels with the plate reader. The dotted and solid circles denote two biological replicates per sample with eight technical replicates each. FRET ratios measured by HyCAP are significantly higher than those determined by the plate reader, owing to differences in the excitation and emission measurement setups. FRET ratio distributions of replicates measured using plate reader (30 replicates each) (D) and HyCAP (E).

    Article Snippet: E. coli BL21 strain , New England Biolabs , C2527H.

    Techniques: Construct, Plasmid Preparation, Expressing, Fluorescence